TY - JOUR
T1 - Kinetics of drug release from a hyaluronan-steroid conjugate investigated by NMR spectroscopy
AU - Taglienti, Anna
AU - Sequi, Paolo
AU - Valentini, Massimiliano
PY - 2009/1/26
Y1 - 2009/1/26
N2 - The methylprednisolone steroid ester of hyaluronan was hydrolyzed under physiological conditions in vitro, and the kinetics of drug release was investigated by NMR spectroscopy. Transverse relaxation times are correlated with the molecular rotational freedom, which undergoes large changes for methylprednisolone when released. Multi-exponential decays were observed, which together with the corresponding population gave valuable insights into the conformational changes that occur in the biopolymer during hydrolysis. The biomaterial exists in aqueous solution in two conformations, 'collapsed' and 'water-exposed', in equilibrium. Under physiological conditions, the methylprednisolone is completely released within 48 h. Transverse relaxation times proved to be an appropriate tool for monitoring the drug release in vitro.
AB - The methylprednisolone steroid ester of hyaluronan was hydrolyzed under physiological conditions in vitro, and the kinetics of drug release was investigated by NMR spectroscopy. Transverse relaxation times are correlated with the molecular rotational freedom, which undergoes large changes for methylprednisolone when released. Multi-exponential decays were observed, which together with the corresponding population gave valuable insights into the conformational changes that occur in the biopolymer during hydrolysis. The biomaterial exists in aqueous solution in two conformations, 'collapsed' and 'water-exposed', in equilibrium. Under physiological conditions, the methylprednisolone is completely released within 48 h. Transverse relaxation times proved to be an appropriate tool for monitoring the drug release in vitro.
KW - Hyaluronan
KW - Methylprednisolone
KW - NMR spectroscopy
KW - Release
KW - Transverse relaxation times
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U2 - 10.1016/j.carres.2008.10.010
DO - 10.1016/j.carres.2008.10.010
M3 - Article
C2 - 18977473
AN - SCOPUS:57749181776
SN - 0008-6215
VL - 344
SP - 245
EP - 249
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 2
ER -