TY - JOUR
T1 - Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis
AU - Balakrishnan, Rengasamy
AU - Elangovan, Namasivayam
AU - Mohankumar, Thangavel
AU - Nataraj, Jagatheesan
AU - Manivasagam, Thamilarasan
AU - Thenmozhi, Arokiasamy Justin
AU - Essa, Musthafa Mohamed
AU - Akbar, Mohammed
AU - Sattar Khan, Mohammed Abdul
N1 - Publisher Copyright:
© 2018 Frontiers in Bioscience. All Rights Reserved.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The present study was carried out to investigatetheneuroprotectiveeffectsofisolongifolene (ILF), a tricyclic sesquiterpene of Murraya koenigii, against rotenone-induced mitochondrial dysfunction. Oxidative stress and apoptosis in a cellular model. SH-SY5Y human neuroblastoma cells were divided into four experimental groups (control, rotenone (100 nM), ILF (10 microM) + rotenone (100 nanoM), ILF 10 microM alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results of the present study showed that the ILF treatment significantly alleviated rotenone-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in SH-SY5Y cells. Moreover, ILF attenuated rotenone induced toxicity by down-regulating Bax, caspases-3, 6, 8 and 9 expression and up-regulating of Bcl-2 expression. Furthermore regulation of p-P13K, p-AKT and p-GSK-3 beta expression by ILF, clearly confirmed its protective effects. Taken together, our results suggested that ILF attenuated rotenone-induced oxidative stress, mitochondrial dysfunction and apoptosis through the regulation of P13K/AKT/GSK-3 beta signaling pathways. However further pre-clinical studies are warranted in rodents to use ILF as a promising therapeutic agent for PD in future.
AB - The present study was carried out to investigatetheneuroprotectiveeffectsofisolongifolene (ILF), a tricyclic sesquiterpene of Murraya koenigii, against rotenone-induced mitochondrial dysfunction. Oxidative stress and apoptosis in a cellular model. SH-SY5Y human neuroblastoma cells were divided into four experimental groups (control, rotenone (100 nM), ILF (10 microM) + rotenone (100 nanoM), ILF 10 microM alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results of the present study showed that the ILF treatment significantly alleviated rotenone-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in SH-SY5Y cells. Moreover, ILF attenuated rotenone induced toxicity by down-regulating Bax, caspases-3, 6, 8 and 9 expression and up-regulating of Bcl-2 expression. Furthermore regulation of p-P13K, p-AKT and p-GSK-3 beta expression by ILF, clearly confirmed its protective effects. Taken together, our results suggested that ILF attenuated rotenone-induced oxidative stress, mitochondrial dysfunction and apoptosis through the regulation of P13K/AKT/GSK-3 beta signaling pathways. However further pre-clinical studies are warranted in rodents to use ILF as a promising therapeutic agent for PD in future.
KW - Apoptosis
KW - Isolongifolene
KW - Mitochondrial Dysfunction
KW - Oxidative Stress
KW - Rotenone
KW - Signaling Pathway
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U2 - 10.2741/s513
DO - 10.2741/s513
M3 - Article
C2 - 28930531
AN - SCOPUS:85037977952
SN - 1945-0516
VL - 10
SP - 248
EP - 261
JO - Frontiers in Bioscience - Scholar
JF - Frontiers in Bioscience - Scholar
IS - 2
ER -