Manuscripts are known for their historical role as explanatory drawings for medical and literary manuscripts. However, the Muslim artist had initially recourse to this Art to speak about his reality including the daily life aspects and his spiritual world. Despite the richness of Islamic manuscript paintings and the prevalence of this Art practice by Muslim artists throughout several periods and eras, both the public and scientific interest are still very limited. This current research aims to determine the artistic and aesthetic values of the manuscript paintings in both Arabic and Persian schools. The goal is to contribute to appreciating manuscript paintings, revitalize the interest and develop their aesthetic and artistic aspects. Through this research we also discuss and analyze the differences between Arabic and Persian schools through selected models from both of them.