Insights into the molecular interaction between sucrose and α-chymotrypsin

Sadegh Farhadian, Behzad Shareghi, Lida Momeni, Osama K. Abou-Zied, Vladimir A. Sirotkin, Masanori Tachiya, Ali A. Saboury*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

39 اقتباسات (Scopus)

ملخص

One of the most important purposes of enzyme engineering is to increase the thermal and kinetic stability of enzymes, which is an important factor for using enzymes in industry. The purpose of the present study is to achieve a higher thermal stability of α-chymotrypsin (α-Chy) by modification of the solvent environment. The influence of sucrose was investigated using thermal denaturation analysis, fluorescence spectroscopy, circular dichroism, molecular docking and molecular dynamics (MD) simulations. The results point to the effect of sucrose in enhancing the α-Chy stability. Fluorescence spectroscopy revealed one binding site that is dominated by static quenching. Molecular docking and MD simulation results indicate that hydrogen bonding and van der Waals forces play a major role in stabilizing the complex. Tm of this complex was enhanced due to the higher H-bond formation and the lower surface hydrophobicity after sucrose modification. The results show the ability of sucrose in protecting the native structural conformation of α-Chy. Sucrose was preferentially excluded from the surface of α-Chy which is explained by the higher tendency of water toward favorable interactions with the functional groups of α-Chy than with sucrose.

اللغة الأصليةEnglish
الصفحات (من إلى)950-960
عدد الصفحات11
دوريةInternational Journal of Biological Macromolecules
مستوى الصوت114
المعرِّفات الرقمية للأشياء
حالة النشرPublished - يوليو 15 2018

ASJC Scopus subject areas

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