Influence of diallyl disulphide on temporal patterns of liver marker enzymes in experimental hepatocarcinogenesis in rats

The present study aims to investigate the effects of diallyl disulphide (DADS) on the temporal patterns of tumor marker enzymes, such as serum alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), and γ-glutamyl transpeptidase (GGT) during N-nitrosodiethylamine (NDEA) induced hepatocarcinogenesis in rats. The acrophase of all marker enzymes was found to be delayed in NDEA-treated rats. The increased mesor, altered amplitude and acrophase of these markers indicated the index of liver damage. Oral treatment of DADS results in normalization of the altered rhythms of these tumor markers (compared with controls) by its anticarcinogenic and antiproliferative effects. Furthermore, this study indicates the necessity of detailed investigation to reveal the temporal interplay between the central biological clock (suprachiasmatic nucleus), peripheral tissue (liver) based oscillators and cancer processes.