Immature and mature human astrovirus: Structure, conformational changes, and similarities to hepatitis e virus

Kelly A. Dryden, Mariana Tihova, Norbert Nowotny, Suzanne M. Matsui, Ernesto Mendez, Mark Yeager*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

51 اقتباسات (Scopus)

ملخص

Human astroviruses (HAstVs) are a major cause of gastroenteritis. HAstV assembles from the structural protein VP90 and undergoes a cascade of proteolytic cleavages. Cleavage to VP70 is required for release of immature particles from cells, and subsequent cleavage by trypsin confers infectivity. We used electron cryomicroscopy and icosahedral image analysis to determine the first experimentally derived, three-dimensional structures of an immature VP70 virion and a fully proteolyzed, infectious virion. Both particles display T = 3 icosahedral symmetry and nearly identical solid capsid shells with diameters of ∼ 350 Å. Globular spikes emanate from the capsid surface, yielding an overall diameter of ∼ 440 Å. While the immature particles display 90 dimeric spikes, the mature capsid only displays 30 spikes, located on the icosahedral 2-fold axes. Loss of the 60 peripentonal spikes likely plays an important role in viral infectivity. In addition, immature HAstV bears a striking resemblance to the structure of hepatitis E virus (HEV)-like particles, as previously predicted from structural similarity of the crystal structure of the astrovirus spike domain with the HEV P-domain [Dong, J., Dong, L., Méndez, E. & Tao, Y. (2011). Crystal structure of the human astrovirus capsid spike. Proc. Natl. Acad. Sci. USA 108, 12681-12686]. Similarities between their capsid shells and dimeric spikes and between the sequences of their capsid proteins suggest that these viral families are phylogenetically related and may share common assembly and activation mechanisms.

اللغة الأصليةEnglish
الصفحات (من إلى)650-658
عدد الصفحات9
دوريةJournal of Molecular Biology
مستوى الصوت422
رقم الإصدار5
المعرِّفات الرقمية للأشياء
حالة النشرPublished - أكتوبر 5 2012
منشور خارجيًانعم

ASJC Scopus subject areas

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