TY - JOUR
T1 - Identification and characterization of an Onchocerca volvulus cDNA clone encoding a microfilarial surface-associated antigen
AU - Lustigman, Sara
AU - Brotman, Betsy
AU - Johnson, Eugene H.
AU - Smith, Andrea B.
AU - Huima, Tellervo
AU - Prince, Alfred M.
N1 - Funding Information:
We thankD r. Albert E. Biancof or the gift of rabbit antiserumra iseda gainstO . lienalis microfilariaeD,r . Hugh Taylorf or serumfr om his Ivermectisnt udyin chimpanzeeDsr, . Ross Coppel for computear nalysesa nd providing the pGEX-1N plasmida nd Ms. P. McCor-mack for technicaal ssistanceW. e are also gratefutlo Dr. F. Perlerf or commentosn the manuscripta,n d Ms. JacquelynA ndersonfo r typingt hemanuscript. This study was supportedb y grantsf rom UNDP, World Bank, WHO SpecialP rogram for Researcahn dT rainingin TropicalD iseases and the Edna McConnellC lark Foundation.
PY - 1992/1
Y1 - 1992/1
N2 - The identification and characterization of a recombinant cDNA clone (OV103) expressing a microfilarial surface-associated antigen of Onchocerca volvulus is described. OV103 was identified and isolated from a λgt11 cDNA expression library derived from adult O. volvulus mRNA using a chimpanzee antiserum, taken 2 years after infection with third-stage larvae of O. volvulus. The cDNA clone encodes a 12.5-kDa protein that corresponds to a 15-kDa parasite protein present in microfilariae and adult female worms. The antigen encoded by this clone is located in the basal layer of the cuticle and the hypodermis of the female adult worm, and on the surface of microfilariae. OV103 fusion polypeptide is recognized only by some sera from onchocerciasis infected subjects (57%), but more significantly (89%) by sera from individuals that have low levels of patent infection. In addition, the antibody response to this protein developed before appearance of microfilariae in the skin of chimpanzees that had developed non-patent or low level patent infections, while the antibody response in chimpanzees with high levels of microfilariae appeared later at the time of appearance of microfilariae. Preliminary experiments indicated that affinity purified antibodies directed against OV103 fusion polypeptide mediated killing of nodular microfilariae in vitro in the presence of normal peripheral blood granulocytes.
AB - The identification and characterization of a recombinant cDNA clone (OV103) expressing a microfilarial surface-associated antigen of Onchocerca volvulus is described. OV103 was identified and isolated from a λgt11 cDNA expression library derived from adult O. volvulus mRNA using a chimpanzee antiserum, taken 2 years after infection with third-stage larvae of O. volvulus. The cDNA clone encodes a 12.5-kDa protein that corresponds to a 15-kDa parasite protein present in microfilariae and adult female worms. The antigen encoded by this clone is located in the basal layer of the cuticle and the hypodermis of the female adult worm, and on the surface of microfilariae. OV103 fusion polypeptide is recognized only by some sera from onchocerciasis infected subjects (57%), but more significantly (89%) by sera from individuals that have low levels of patent infection. In addition, the antibody response to this protein developed before appearance of microfilariae in the skin of chimpanzees that had developed non-patent or low level patent infections, while the antibody response in chimpanzees with high levels of microfilariae appeared later at the time of appearance of microfilariae. Preliminary experiments indicated that affinity purified antibodies directed against OV103 fusion polypeptide mediated killing of nodular microfilariae in vitro in the presence of normal peripheral blood granulocytes.
KW - Immunoelectron microscopy
KW - Killing of microfilariae
KW - Microfilaria
KW - Onchocerca volvulus
KW - Recombinant antigen
KW - Surface-associated antigen
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U2 - 10.1016/0166-6851(92)90246-G
DO - 10.1016/0166-6851(92)90246-G
M3 - Article
C2 - 1542318
AN - SCOPUS:0026555237
SN - 0166-6851
VL - 50
SP - 79
EP - 93
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -