TY - JOUR
T1 - HPLC coupled to UV-vis detection for quantitative determination of phenolic compounds and caffeine in different brands of coffee in the Algerian market
AU - Belguidoum, K.
AU - Amira-Guebailia, H.
AU - Boulmokh, Y.
AU - Houache, O.
PY - 2014/7
Y1 - 2014/7
N2 - HPLC coupled to UV-vis detection was used for the analysis of eight phenolic acids, three flavonoids, and caffeine in sixteen coffee samples (roasted, green and instant) collected from the Algerian market. The following parameters were taken into account for coffee analyses: packaging, roasting degree, grain size, instantaneity, and decaffeination.The total polyphenols and caffeine levels in coffee extracts were found to vary from 12.37. ±. 0.55 to 200.08. ±. 6.47 mg/l, and 38.00. ±. 1.89 to 136.00. ±. 6.45 mg/l, respectively.Evaluation of chromatographic performance showed excellent reproducibility, resolution, selectivity, and reasonable peak symmetry. The limit of detection (LOD) ranged from 0.75 to 14.79. μg/l, while the limit of quantification (LOQ) ranged from 2.26 to 44.44. μg/l. The separation of all compounds was achieved within 13. min.
AB - HPLC coupled to UV-vis detection was used for the analysis of eight phenolic acids, three flavonoids, and caffeine in sixteen coffee samples (roasted, green and instant) collected from the Algerian market. The following parameters were taken into account for coffee analyses: packaging, roasting degree, grain size, instantaneity, and decaffeination.The total polyphenols and caffeine levels in coffee extracts were found to vary from 12.37. ±. 0.55 to 200.08. ±. 6.47 mg/l, and 38.00. ±. 1.89 to 136.00. ±. 6.45 mg/l, respectively.Evaluation of chromatographic performance showed excellent reproducibility, resolution, selectivity, and reasonable peak symmetry. The limit of detection (LOD) ranged from 0.75 to 14.79. μg/l, while the limit of quantification (LOQ) ranged from 2.26 to 44.44. μg/l. The separation of all compounds was achieved within 13. min.
KW - Caffeine
KW - Catechin
KW - Coffee
KW - HPLC
KW - Phenolic acids
KW - Polyphenols
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U2 - 10.1016/j.jtice.2014.03.014
DO - 10.1016/j.jtice.2014.03.014
M3 - Article
AN - SCOPUS:84903398139
SN - 1876-1070
VL - 45
SP - 1314
EP - 1320
JO - Journal of the Taiwan Institute of Chemical Engineers
JF - Journal of the Taiwan Institute of Chemical Engineers
IS - 4
ER -