TY - JOUR
T1 - High-performance liquid chromatography determination of anilines with fluorescent detection and pre-column derivatization
AU - Al-Kindy, Salma M.Z.
AU - Al-Kalbani, Azza
AU - Al-Harasi, Ahmed F.
AU - Suliman, Fakhr Eldin O.
AU - Al-Lawati, Haider J.
AU - Al-Hamadi, Abdalla
N1 - Funding Information:
Financial support from SQU, grant #IG/SCI/CHEM/09/01, is gratefully acknowledged.
PY - 2013/1/1
Y1 - 2013/1/1
N2 - A simple, sensitive, and rapid reverse-phase high-performance liquid chromatography (RP-HPLC) method for the determination of anilines in water is proposed. The use of 2,7-diethylamino-2-oxo-2H-chromen-3-yl-benzothiazole-6- sulfonylchloride (coumarin 6-SO2Cl) as a fluorigenic-labeling reagent was investigated. The label reacted with aniline within 30 min under mild conditions (ambient temperature, pH 9.0) to give sulfonamides that were separated by RP-HPLC employing fluorescence detection with an excitation wavelength of 470 nm and an emission wavelength of 520 nm. The optimum conditions for fluorescence, derivatization, and chromatographic separation were established. The calibration curves were linear for the range 0-800 ppb. The proposed method was applied for the determination of anilines in spiked drinking water samples and irrigation water samples with recoveries of 90.0-103.9% and relative standard deviations of 1.2-4.7%, respectively. This method showed good accuracy and repeatability that can be used for the quantification of aniline in real samples.
AB - A simple, sensitive, and rapid reverse-phase high-performance liquid chromatography (RP-HPLC) method for the determination of anilines in water is proposed. The use of 2,7-diethylamino-2-oxo-2H-chromen-3-yl-benzothiazole-6- sulfonylchloride (coumarin 6-SO2Cl) as a fluorigenic-labeling reagent was investigated. The label reacted with aniline within 30 min under mild conditions (ambient temperature, pH 9.0) to give sulfonamides that were separated by RP-HPLC employing fluorescence detection with an excitation wavelength of 470 nm and an emission wavelength of 520 nm. The optimum conditions for fluorescence, derivatization, and chromatographic separation were established. The calibration curves were linear for the range 0-800 ppb. The proposed method was applied for the determination of anilines in spiked drinking water samples and irrigation water samples with recoveries of 90.0-103.9% and relative standard deviations of 1.2-4.7%, respectively. This method showed good accuracy and repeatability that can be used for the quantification of aniline in real samples.
KW - anilines
KW - coumarin 6-SOCL
KW - fluorescence detection
KW - high-performance liquid chromatography (HPLC)
KW - pre-column derivatization
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U2 - 10.1080/10739149.2012.721108
DO - 10.1080/10739149.2012.721108
M3 - Article
AN - SCOPUS:84873684892
SN - 1073-9149
VL - 41
SP - 48
EP - 59
JO - Instrumentation Science and Technology
JF - Instrumentation Science and Technology
IS - 1
ER -