TY - JOUR
T1 - Heavy metal resistant of E. coli isolated from wastewater sites in Assiut City, Egypt
AU - Abskharon, R. N.N.
AU - Hassan, S. H.A.
AU - Gad El-Rab, S. M.F.
AU - Shoreit, A. A.M.
PY - 2008/9
Y1 - 2008/9
N2 - Twelve isolates of E. coli were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weights 27 and 65 kb for hexa- and trivalent chromium. It was identified as E. coli ASU 7. Its minimal inhibitory concentration (MIC) for Cu 2+, Co 2+, Ni 2+, Zn 2+, Cr 6+, Cr 3+, Cd 2+ and Pb 2+ were 1.57, 2.55, 1.7, 9.17, 0.48, 7.69, 4.4 and 3.1 mM, respectively. Growth kinetics was determined under Cr 6+ and Cr 3+ stress. SDS-PAGE of protein profile shows that 10 ppm (0.19 mM) of Cr 6+ induces new protein with molecular weight 23 kDa.
AB - Twelve isolates of E. coli were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weights 27 and 65 kb for hexa- and trivalent chromium. It was identified as E. coli ASU 7. Its minimal inhibitory concentration (MIC) for Cu 2+, Co 2+, Ni 2+, Zn 2+, Cr 6+, Cr 3+, Cd 2+ and Pb 2+ were 1.57, 2.55, 1.7, 9.17, 0.48, 7.69, 4.4 and 3.1 mM, respectively. Growth kinetics was determined under Cr 6+ and Cr 3+ stress. SDS-PAGE of protein profile shows that 10 ppm (0.19 mM) of Cr 6+ induces new protein with molecular weight 23 kDa.
KW - Antibiotics resistance
KW - E. coli
KW - Heavy metal resistance
KW - Wastewater
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U2 - 10.1007/s00128-008-9494-6
DO - 10.1007/s00128-008-9494-6
M3 - Article
C2 - 18584108
AN - SCOPUS:49349110248
SN - 0007-4861
VL - 81
SP - 309
EP - 315
JO - Bulletin of Environmental Contamination and Toxicology
JF - Bulletin of Environmental Contamination and Toxicology
IS - 3
ER -