TY - JOUR
T1 - Gum Arabic Supplementation Suppresses Colonic Fibrosis after Acute Colitis by Reducing Transforming Growth Factor β1 Expression
AU - Al-Araimi, Amna
AU - Al Kindi, Ishraq A.
AU - Bani Oraba, Asma
AU - Alkharusi, Amira
AU - Ali, Badreldin H.
AU - Zadjali, Razan
AU - Al Sinawi, Shadia
AU - Al-Haddabi, Ibrahim
AU - Zadjali, Fahad
N1 - Funding Information:
This study was funded by a Sultan Qaboos University strategic grant and an internal grant (IG/MED/BIOC/13/01 and SR/SQU/MED/BIOC/13/01).
Publisher Copyright:
© Copyright 2021, Mary Ann Liebert, Inc., publishers, and Korean Society of Food Science and Nutrition 2021.
PY - 2021/12
Y1 - 2021/12
N2 - Ulcerative colitis is a chronic inflammation of the colonic mucosa. Gum Arabic (GA) has been reported to exert anti-inflammatory and antifibrotic activity. This study aimed to evaluate the effect of GA on disease activity in an experimental model of colitis. Dextran sodium sulfate (DSS) was used to induce colitis in C57BL/6 mice and the animals were then switched to normal drinking water to monitor recovery. Mice received 140 g/L GA before (pre-GA group) or after (post-GA group) induction of colitis. Disease activity and recovery were assessed by changes in body weight, disease activity index (DAI), and histological assessment. Gene expression of proinflammatory, anti-inflammatory, and fibrotic markers was measured in colonic tissues. Mice in the pre-GA group showed an increase in body weight, with no differences in DAI scores, during the recovery phase and had lower histological colitis scores than mice in the post-GA group, which showed higher DAI and histological scores during the recovery phase. During the recovery phase, mice in the pre-GA group showed increased expression of proinflammatory markers, while gene expression of the fibrotic markers, transforming growth factor β1 (TGFβ1) and procollagen I, was reduced. The reduced fibrotic marker expression was associated with reduced collagen staining and increased epithelial cell proliferation. Administration of GA had protective and alleviative effects on the severity of DSS-induced colitis, with a reduction in colonic fibrosis and TGFβ1 expression. These data warrant further in vitro and in vivo investigations on the effect of GA on fibroblast activity.
AB - Ulcerative colitis is a chronic inflammation of the colonic mucosa. Gum Arabic (GA) has been reported to exert anti-inflammatory and antifibrotic activity. This study aimed to evaluate the effect of GA on disease activity in an experimental model of colitis. Dextran sodium sulfate (DSS) was used to induce colitis in C57BL/6 mice and the animals were then switched to normal drinking water to monitor recovery. Mice received 140 g/L GA before (pre-GA group) or after (post-GA group) induction of colitis. Disease activity and recovery were assessed by changes in body weight, disease activity index (DAI), and histological assessment. Gene expression of proinflammatory, anti-inflammatory, and fibrotic markers was measured in colonic tissues. Mice in the pre-GA group showed an increase in body weight, with no differences in DAI scores, during the recovery phase and had lower histological colitis scores than mice in the post-GA group, which showed higher DAI and histological scores during the recovery phase. During the recovery phase, mice in the pre-GA group showed increased expression of proinflammatory markers, while gene expression of the fibrotic markers, transforming growth factor β1 (TGFβ1) and procollagen I, was reduced. The reduced fibrotic marker expression was associated with reduced collagen staining and increased epithelial cell proliferation. Administration of GA had protective and alleviative effects on the severity of DSS-induced colitis, with a reduction in colonic fibrosis and TGFβ1 expression. These data warrant further in vitro and in vivo investigations on the effect of GA on fibroblast activity.
KW - fibrosis
KW - gum Arabic
KW - inflammatory bowel disease
KW - ulcerative colitis
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U2 - 10.1089/jmf.2021.0007
DO - 10.1089/jmf.2021.0007
M3 - Article
C2 - 34704833
AN - SCOPUS:85122126021
SN - 1096-620X
VL - 24
SP - 1255
EP - 1263
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 12
ER -