Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Jørgen Stenvik, Mette Serine Wesmajervi, Børge Damsgård, Madjid Delghandi*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةArticleمراجعة النظراء

27 اقتباسات (Scopus)

ملخص

The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.

اللغة الأصليةEnglish
الصفحات (من إلى)272-275
عدد الصفحات4
دوريةMolecular Ecology Notes
مستوى الصوت6
رقم الإصدار1
المعرِّفات الرقمية للأشياء
حالة النشرPublished - مارس 2006
منشور خارجيًانعم

ASJC Scopus subject areas

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بصمة

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