ملخص
The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.
اللغة الأصلية | English |
---|---|
الصفحات (من إلى) | 272-275 |
عدد الصفحات | 4 |
دورية | Molecular Ecology Notes |
مستوى الصوت | 6 |
رقم الإصدار | 1 |
المعرِّفات الرقمية للأشياء | |
حالة النشر | Published - مارس 2006 |
منشور خارجيًا | نعم |
ASJC Scopus subject areas
- ???subjectarea.asjc.1300.1303???
- ???subjectarea.asjc.2300.2303???
- ???subjectarea.asjc.1300.1300???