TY - JOUR
T1 - Fluorescence quenching studies of nitrated polycyclic aromatic hydrocarbons
AU - Al-Kindy, Salma M.Z.
AU - Miller, James N.
PY - 2011
Y1 - 2011
N2 - The analysis of nitrated polycyclic aromatic hydrocarbons (NPAHs) is of great importance because of the mutagenicity and possible carcinogenic activity of these compounds, which are distributed widely in the environment. Nitro-substituents in aromatic compounds are known to quench fluorescence and NPAHs have no intrinsic fluorescence, but they can be determined using their quenching effects on other fluorophores. The quenching effects of several important NPAHs on 1,2,3,4- tetrahydro-1-naphthol,5,6,7,8-tetrahydro-1-naphthol, 4-(2-hydroxy-4-sulfo-1-naphthylazo)-2-naphthalene carboxylic acid and 7-amino-4-methyl coumarin have been studied. The singlet emission of these fluorophores is efficiently quenched by all the NPAHs, the quenching following the Stern-Volmer relationship. Quenching constants and the limits of detection and linear ranges of the quenchers have been determined in each case: the limits of detection are ca 1 μm.
AB - The analysis of nitrated polycyclic aromatic hydrocarbons (NPAHs) is of great importance because of the mutagenicity and possible carcinogenic activity of these compounds, which are distributed widely in the environment. Nitro-substituents in aromatic compounds are known to quench fluorescence and NPAHs have no intrinsic fluorescence, but they can be determined using their quenching effects on other fluorophores. The quenching effects of several important NPAHs on 1,2,3,4- tetrahydro-1-naphthol,5,6,7,8-tetrahydro-1-naphthol, 4-(2-hydroxy-4-sulfo-1-naphthylazo)-2-naphthalene carboxylic acid and 7-amino-4-methyl coumarin have been studied. The singlet emission of these fluorophores is efficiently quenched by all the NPAHs, the quenching following the Stern-Volmer relationship. Quenching constants and the limits of detection and linear ranges of the quenchers have been determined in each case: the limits of detection are ca 1 μm.
KW - Stern -Volmer
KW - fluorescence quenching
KW - fluorophores
KW - nitrated polycyclic aromatic hydrocarbons
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U2 - 10.1002/bio.1205
DO - 10.1002/bio.1205
M3 - Article
C2 - 20354970
AN - SCOPUS:79954596927
SN - 1522-7235
VL - 26
SP - 148
EP - 152
JO - Luminescence
JF - Luminescence
IS - 2
ER -