Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions

N. Idayu Zahid, Osama K. Abou-Zied, Rauzah Hashim

نتاج البحث

1 اقتباس (Scopus)

ملخص

Water-driven self-assembly of lipids displays a variety of liquid crystalline phases that are crucial for membrane functions. In this work, we characterized the temperature-induced phase transitions in aqueous self-assembly systems using steady-state and time-resolved fluorescence measurements. The polar head group region was investigated using tryptophan (Trp) and two of its ester derivatives, and the hydrophobic tail region was probed using pyrene. The spectral changes in tryptophan and pyrene were used as a benchmark to estimate the polarity of the head group region and the tail region, respectively. A basic medium was detected and estimated for the polar region in the inverse cubic phase of a Guerbet glycolipid and was attributed to the structural effect of the narrow nanochannels. All the studied lipid compositions show completely reversible temperature-induced phase transitions, reflecting the thermodynamic equilibrium structures of their mesophases. The results reveal a large degree of heterogeneity and flexibility of the lipid self-assembly which may be crucial for carrying out different biological functions.

اللغة الأصليةEnglish
عنوان منشور المضيفReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII
المحررونRamesh Raghavachari, Samuel Achilefu
ناشرSPIE
رقم المعيار الدولي للكتب (الإلكتروني)9781628414295
المعرِّفات الرقمية للأشياء
حالة النشرPublished - 2015
الحدثReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII - San Francisco
المدة: فبراير ٨ ٢٠١٥فبراير ١٠ ٢٠١٥

سلسلة المنشورات

الاسمProgress in Biomedical Optics and Imaging - Proceedings of SPIE
مستوى الصوت9339
رقم المعيار الدولي للدوريات (المطبوع)1605-7422

Other

OtherReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII
الدولة/الإقليمUnited States
المدينةSan Francisco
المدة٢/٨/١٥٢/١٠/١٥

ASJC Scopus subject areas

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