TY - JOUR
T1 - Evaluation of anti-ds DNA antibodies in anti-nuclear antibody positive Omani patients
AU - Alnaqdy, Adel
AU - Al-Busaidy, Juma
AU - Hassan, Batool
PY - 2007/4
Y1 - 2007/4
N2 - Objective: To study the correlation between enzyme linked immunosorbent assay (ELISA) and immunofluoresencent (IF) anti-dsDNA antibody measurement in different diseases. Patients: One hundred and forty sera from patients with systemic lupus erythematosus (SLE, n=40), rheumatoid arthritis (RA, n=30), disease control (n=40) and from healthy control subjects (n=30) were included. Results: Using the ELISA, serum anti-dsDNA was detected in 24/40 (60%) SLE, 5/30 (16.6%) RA, 9/40 (22.5%) disease controls and 1/30 (3.3%) of normal healthy controls. When IF assay was employed, anti-dsDNA antibodies were detected in 16/40 (40%) SLE, 3/30 (10%) RA, 5/40 (12.5%) disease controls but none in the normal subjects. Conclusion: These results suggest that both ELISA and IF techniques can be used for the measurement of anti-dsDNA antibodies in clinical laboratories and, these antibodies are not unique to SLE.
AB - Objective: To study the correlation between enzyme linked immunosorbent assay (ELISA) and immunofluoresencent (IF) anti-dsDNA antibody measurement in different diseases. Patients: One hundred and forty sera from patients with systemic lupus erythematosus (SLE, n=40), rheumatoid arthritis (RA, n=30), disease control (n=40) and from healthy control subjects (n=30) were included. Results: Using the ELISA, serum anti-dsDNA was detected in 24/40 (60%) SLE, 5/30 (16.6%) RA, 9/40 (22.5%) disease controls and 1/30 (3.3%) of normal healthy controls. When IF assay was employed, anti-dsDNA antibodies were detected in 16/40 (40%) SLE, 3/30 (10%) RA, 5/40 (12.5%) disease controls but none in the normal subjects. Conclusion: These results suggest that both ELISA and IF techniques can be used for the measurement of anti-dsDNA antibodies in clinical laboratories and, these antibodies are not unique to SLE.
KW - Anti-dsDNA antibodies
KW - Crithidia luciliae
KW - ELISA
KW - Heumatoid arthritis
KW - Systemic lupus erythematosus
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M3 - Article
AN - SCOPUS:34249728425
SN - 1682-024X
VL - 23
SP - 211
EP - 215
JO - Pakistan Journal of Medical Sciences
JF - Pakistan Journal of Medical Sciences
IS - 2
ER -