Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Karabi Datta; Jumin Tu; Norman Oliva; Isabelita Ona; Rethinasamy Velazhahan; Twng Wah Mew; Subbaratnam Muthukrishnan; Swapan K. Datta

doi:10.1016/S0168-9452(00)00413-1

Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Karabi Datta, Jumin Tu, Norman Oliva, Isabelita Ona, Rethinasamy Velazhahan, Twng Wah Mew, Subbaratnam Muthukrishnan, Swapan K. Datta^*

^*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلة › Article › مراجعة النظراء

190 اقتباسات (Scopus)

ملخص

Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T₂ generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

اللغة الأصلية	English
الصفحات (من إلى)	405-414
عدد الصفحات	10
دورية	Plant Science
مستوى الصوت	160
رقم الإصدار	3
المعرِّفات الرقمية للأشياء	https://doi.org/10.1016/S0168-9452(00)00413-1
حالة النشر	Published - فبراير 5 2001
منشور خارجيًا	نعم

ASJC Scopus subject areas

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الوصول إلى المستند

10.1016/S0168-9452(00)00413-1

الملفات والروابط الأخرى

Link to publication in Scopus

قم بذكر هذا

Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars. / Datta, Karabi; Tu, Jumin; Oliva, Norman وآخرون.
في: Plant Science, المجلد 160, رقم 3, ٠٥.٠٢.٢٠٠١, صفحة 405-414.

نتاج البحث: المساهمة في مجلة › Article › مراجعة النظراء

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title = "Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars",

abstract = "Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.",

keywords = "Chitinase, Enhanced resistance, Sheath blight, Transgenic rice",

author = "Karabi Datta and Jumin Tu and Norman Oliva and Isabelita Ona and Rethinasamy Velazhahan and Mew, {Twng Wah} and Subbaratnam Muthukrishnan and Datta, {Swapan K.}",

note = "Funding Information: We thank BMZ (GTZ, Germany) and the Rockefeller Foundation, USA, for financial assistance. Thanks are due to Bill Hardy for editorial assistance.",

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doi = "10.1016/S0168-9452(00)00413-1",

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T1 - Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

AU - Datta, Karabi

AU - Tu, Jumin

AU - Oliva, Norman

AU - Ona, Isabelita

AU - Velazhahan, Rethinasamy

AU - Mew, Twng Wah

AU - Muthukrishnan, Subbaratnam

AU - Datta, Swapan K.

N1 - Funding Information: We thank BMZ (GTZ, Germany) and the Rockefeller Foundation, USA, for financial assistance. Thanks are due to Bill Hardy for editorial assistance.

PY - 2001/2/5

Y1 - 2001/2/5

N2 - Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

AB - Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

KW - Chitinase

KW - Enhanced resistance

KW - Sheath blight

KW - Transgenic rice

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Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

ملخص

ASJC Scopus subject areas

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