TY - JOUR
T1 - Endothelial cells freshly isolated from resistance-sized pulmonary arteries possess a unique K+ current profile
AU - Hogg, Dayle S.
AU - Albarwani, Sulayma
AU - Davies, Andrew R.L.
AU - Kozlowski, Roland Z.
N1 - Funding Information:
This work was supported by the British Heart Foundation and the Wellcome Trust. D.S.H. is a BHF student and R.Z.K. is a BHF lecturer. The authors acknowledge the help of Dr. Christine Wilson who initiated the immunocytochemical studies detailed here.
PY - 1999/9/24
Y1 - 1999/9/24
N2 - We have, for the first time, developed a reliable method for freshly isolating viable endothelial cells from resistance-sized rat pulmonary arteries. The endothelial origin of these cells was confirmed using indirect immunofluorescence, utilizing fluorescently labeled low-density lipoprotein. Biophysical and pharmacological patch-clamp experiments conducted under quasiphysiological cationic gradients revealed that these cells had a mean resting membrane potential of ~ -38 mV and displayed a delayed-rectifying K+ current. Immunohistochemical staining of rat lung cross-sections revealed an abundance of K(V)1.5 channel protein in pulmonary endothelium. This is the first report of a delayed-rectifying K+ current in endothelial cells of resistance-sized pulmonary arteries. Since changes in membrane potential associated with K+ channel activity affect release of vasoactive substances from endothelial cells, this finding has important implications for understanding the mechanisms underlying control of pulmonary vascular tone.
AB - We have, for the first time, developed a reliable method for freshly isolating viable endothelial cells from resistance-sized rat pulmonary arteries. The endothelial origin of these cells was confirmed using indirect immunofluorescence, utilizing fluorescently labeled low-density lipoprotein. Biophysical and pharmacological patch-clamp experiments conducted under quasiphysiological cationic gradients revealed that these cells had a mean resting membrane potential of ~ -38 mV and displayed a delayed-rectifying K+ current. Immunohistochemical staining of rat lung cross-sections revealed an abundance of K(V)1.5 channel protein in pulmonary endothelium. This is the first report of a delayed-rectifying K+ current in endothelial cells of resistance-sized pulmonary arteries. Since changes in membrane potential associated with K+ channel activity affect release of vasoactive substances from endothelial cells, this finding has important implications for understanding the mechanisms underlying control of pulmonary vascular tone.
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U2 - 10.1006/bbrc.1999.1338
DO - 10.1006/bbrc.1999.1338
M3 - Article
C2 - 10491306
AN - SCOPUS:0033600710
SN - 0006-291X
VL - 263
SP - 405
EP - 409
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -