TY - JOUR
T1 - Development of new formulations of Bacillus subtilis for management of tomato damping-off caused by Pythium aphanidermatum
AU - Jayaraj, J.
AU - Radhakrishnan, N. V.
AU - Kannan, R.
AU - Sakthivel, K.
AU - Suganya, D.
AU - Venkatesan, S.
AU - Velazhahan, R.
N1 - Funding Information:
This study was supported by the Tamilnadu State Council for Science and Technology, Government of Tamilnadu, Chennai, India, through a research grant to J.J. Due thanks to Professor Zamir K. Punja (Department of Biological Sciences, SFU) for critical reading of the manuscript.
PY - 2005/2
Y1 - 2005/2
N2 - Formulations of a strain of Bacillus subtilis AUBS-1 inhibitory to the growth of the damping-off pathogen, Pythium aphanidermatum, were developed for seed treatment. The formulations included a talc-based powder, lignite-based powder, lignite + fly ash-based powder, wettable powder, bentonite-paste, polyethylene glycol (PEG) paste and a water-dispersible tablet. Formulations were stored at room temperature for 2 years and frequently sampled to test their shelf life. Populations of bacteria in the formulations were stable for up to 2 years storage at room temperature (28°C). Viability of propagules in lignite, lignite + fly ash, bentonite paste, wettable powder and water dispersible tablet formulations was 100% for up to 1 year. However, the viability of propagules was significantly reduced in talc, wettable powder, PEG paste and tablet formulations beyond 1 year of storage. Seed treatment of tomato with these formulations resulted in effective control of damping-off caused by P. aphanidermatum, and also enhanced plant biomass under glasshouse and field conditions. Active rhizosphere colonization by the bacterium was observed on tomato plants grown from seeds treated with the above formulations.
AB - Formulations of a strain of Bacillus subtilis AUBS-1 inhibitory to the growth of the damping-off pathogen, Pythium aphanidermatum, were developed for seed treatment. The formulations included a talc-based powder, lignite-based powder, lignite + fly ash-based powder, wettable powder, bentonite-paste, polyethylene glycol (PEG) paste and a water-dispersible tablet. Formulations were stored at room temperature for 2 years and frequently sampled to test their shelf life. Populations of bacteria in the formulations were stable for up to 2 years storage at room temperature (28°C). Viability of propagules in lignite, lignite + fly ash, bentonite paste, wettable powder and water dispersible tablet formulations was 100% for up to 1 year. However, the viability of propagules was significantly reduced in talc, wettable powder, PEG paste and tablet formulations beyond 1 year of storage. Seed treatment of tomato with these formulations resulted in effective control of damping-off caused by P. aphanidermatum, and also enhanced plant biomass under glasshouse and field conditions. Active rhizosphere colonization by the bacterium was observed on tomato plants grown from seeds treated with the above formulations.
KW - Bacillus subtilis
KW - Biocontrol
KW - Damping-off
KW - Formulations
KW - Pythium aphanidermatum
KW - Seed treatment
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U2 - 10.1080/09583150400015920
DO - 10.1080/09583150400015920
M3 - Article
AN - SCOPUS:12544255445
SN - 0958-3157
VL - 15
SP - 55
EP - 65
JO - Biocontrol Science and Technology
JF - Biocontrol Science and Technology
IS - 1
ER -