TY - JOUR
T1 - Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats
AU - Mohd Azamai, Emey Suhana
AU - Sulaiman, Suhaniza
AU - Mohd Habib, Shafina Hanim
AU - Looi, Mee Lee
AU - Das, Srijit
AU - Abdul Hamid, Nor Aini
AU - Wan Ngah, Wan Zurinah
AU - Mohd Yusof, Yasmin Anum
N1 - Funding Information:
‡ Corresponding author * Project supported by Department of Biochemistry, Faculty of Medicine, UKM Medical Center, Universiti Kebangsaan Malaysia, and the Malaysian Ministry of Science and Technological Innovation (MOSTI)
PY - 2009
Y1 - 2009
N2 - Chlorella vulgaris (CV) has been reported to have antioxidant and anticancer properties. We evaluated the effect of CV on apoptotic regulator protein expression in liver cancer-induced rats. Male Wistar rats (200∼250 g) were divided into eight groups: control group (normal diet), CDE group (choline deficient diet supplemented with ethionine in drinking water to induce hepatocarcinogenesis), CV groups with three different doses of CV (50, 150, and 300 mg/kg body weight), and CDE groups treated with different doses of CV (50, 150, and 300 mg/ kg body weight). Rats were sacrificed at various weeks and liver tissues were embedded in paraffin blocks for immunohistochemistry studies. CV, at increasing doses, decreased the expression of anti-apoptotic protein, Bcl-2, but increased the expression of pro-apoptotic protein, caspase 8, in CDE rats, which was correlated with decreased hepatoctyes proliferation and increased apoptosis as determined by bromodeoxy-uridine (BrdU) labeling and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, respectively. Our study shows that CV has definite chemopreventive effect by inducing apoptosis via decreasing the expression of Bcl-2 and increasing the expression of caspase 8 in hepatocarcinogenesis-induced rats.
AB - Chlorella vulgaris (CV) has been reported to have antioxidant and anticancer properties. We evaluated the effect of CV on apoptotic regulator protein expression in liver cancer-induced rats. Male Wistar rats (200∼250 g) were divided into eight groups: control group (normal diet), CDE group (choline deficient diet supplemented with ethionine in drinking water to induce hepatocarcinogenesis), CV groups with three different doses of CV (50, 150, and 300 mg/kg body weight), and CDE groups treated with different doses of CV (50, 150, and 300 mg/ kg body weight). Rats were sacrificed at various weeks and liver tissues were embedded in paraffin blocks for immunohistochemistry studies. CV, at increasing doses, decreased the expression of anti-apoptotic protein, Bcl-2, but increased the expression of pro-apoptotic protein, caspase 8, in CDE rats, which was correlated with decreased hepatoctyes proliferation and increased apoptosis as determined by bromodeoxy-uridine (BrdU) labeling and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, respectively. Our study shows that CV has definite chemopreventive effect by inducing apoptosis via decreasing the expression of Bcl-2 and increasing the expression of caspase 8 in hepatocarcinogenesis-induced rats.
KW - Apoptosis
KW - Bcl-2
KW - Caspase8
KW - Chlorella vulgaris (CV)
KW - Livercancer
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U2 - 10.1631/jzus.B0820168
DO - 10.1631/jzus.B0820168
M3 - Article
C2 - 19198018
AN - SCOPUS:58549097127
SN - 1673-1581
VL - 10
SP - 14
EP - 21
JO - Journal of Zhejiang University: Science B
JF - Journal of Zhejiang University: Science B
IS - 1
ER -