Characterization of mineral phosphate-solubilizing bacteria for enhanced sunflower growth and yield-attributing traits

Muhammad Shahid*, Sohail Hameed, Mohsin Tariq, Mohsin Zafar, Amanat Ali, Naveed Ahmad

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

51 اقتباسات (Scopus)

ملخص

Nineteen isolates of rhizobacteria associated with sunflower (Helianthus annuus L.), collected from various locations in Pakistan, were screened for phosphate solubilization and indole-3-acetic acid (IAA) production. Two potential phosphate-solubilizing bacterial isolates with substantial IAA biosynthesis capacity, Ps-5 and Ss-2, were selected for further study. Based on 16S rRNA gene sequence analysis, isolate Ps-5 was identified as Bacillus sp. and Ss-2 as Alcaligenes faecalis. Both strains were found to be metabolically diverse in terms of the number and amount of different carbon substrates they utilized in the BIOLOG GN2/GP2 microplate assay. High-performance liquid chromatography analysis of the culture supernatant confirmed that Bacillus sp. Ps-5 produced considerable amounts of both lactic and tartaric acids, while A. faecalis Ss-2 secreted only lactic acid. There was a strong positive correlation between phosphate solubilization and organic acid production by both strains. Following inoculation, strain Ps-5 and Ss-2 were found to be good root colonizers and significantly (P ≤ 0.05) increased sunflower growth and phosphorus (P) uptake. However, inoculation had a non-significant (P ≤ 0.05) effect on sunflower yield parameters, including oil contents. Based on these results, we conclude that Ps-5 and Ss-2 are potent plant growth-promoting rhizobacteria strains with the ability to supplement the P requirements of sunflower crops. Further field inoculation studies are needed before these strains can be recommended as bio-inoculants. To the best of our knowledge, this is first report on the association and phytobeneficial potential of A. faecalis with sunflower.

اللغة الأصليةEnglish
الصفحات (من إلى)1525-1536
عدد الصفحات12
دوريةAnnals of Microbiology
مستوى الصوت65
رقم الإصدار3
المعرِّفات الرقمية للأشياء
حالة النشرPublished - سبتمبر 11 2015

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