TY - JOUR
T1 - Carbon nanotubes based electrochemical aptasensing platform for the detection of hydroxylated polychlorinated biphenyl in human blood serum
AU - Pilehvar, Sanaz
AU - Ahmad Rather, Jahangir
AU - Dardenne, Freddy
AU - Robbens, Johan
AU - Blust, Ronny
AU - De Wael, Karolien
N1 - Funding Information:
We are thankful to UA-DOCPRO and BELSPO for financial support (respectively S. Pilehvar and J. Ahmad Rather). We also thank Prof. A. Covaci (UA) for the kind gift of human blood serum samples. Special thanks to Prof. L. Van Vaeck and Y. Vercammen (UA) for AFM imaging and Prof. V. Meynen and M. Kus (LADCA, UA) for performing IR measurements.
PY - 2014/4/15
Y1 - 2014/4/15
N2 - A novel strategy to sense target molecules in human blood serum is achieved by immobilizing aptamers (APTs) on multi-walled carbon nanotubes (MWCNT) modified electrodes. In this work, the aminated aptamer selected for hydroxylated polychlorinated biphenyl (OH-PCB) was covalently immobilized on the surface of the MWCNT-COOH modified glassy carbon electrode through amide linkage. The aptamers function as recognition probes for OH-PCB by the binding induced folding of the aptamer. The developed aptasensing device was characterized by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The aptasensor displayed excellent performance for OH-PCB detection with a linear range from 0.16 to 7.5μM. The sensitivity of the developed aptasensing platform is improved (1×10-8M) compared to the published report (1×10-6M) for the determination of OH-PCB (Turner et al., 2007). The better performance of the sensor is due to the unique platform, i.e. the presence of APTs onto electrodes and the combination with nanomaterials. The aptamer density on the electrode surface was estimated by chronocoulometry and was found to be 1.4×1013moleculescm-2. The validity of the method and applicability of the aptasensor was successfully evaluated by the detection of OH-PCB in a blood serum sample. The described approach for aptasensing opens up new perspectives in the field of biomonitoring providing a device with acceptable stability, high sensitivity, good accuracy and precision.
AB - A novel strategy to sense target molecules in human blood serum is achieved by immobilizing aptamers (APTs) on multi-walled carbon nanotubes (MWCNT) modified electrodes. In this work, the aminated aptamer selected for hydroxylated polychlorinated biphenyl (OH-PCB) was covalently immobilized on the surface of the MWCNT-COOH modified glassy carbon electrode through amide linkage. The aptamers function as recognition probes for OH-PCB by the binding induced folding of the aptamer. The developed aptasensing device was characterized by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The aptasensor displayed excellent performance for OH-PCB detection with a linear range from 0.16 to 7.5μM. The sensitivity of the developed aptasensing platform is improved (1×10-8M) compared to the published report (1×10-6M) for the determination of OH-PCB (Turner et al., 2007). The better performance of the sensor is due to the unique platform, i.e. the presence of APTs onto electrodes and the combination with nanomaterials. The aptamer density on the electrode surface was estimated by chronocoulometry and was found to be 1.4×1013moleculescm-2. The validity of the method and applicability of the aptasensor was successfully evaluated by the detection of OH-PCB in a blood serum sample. The described approach for aptasensing opens up new perspectives in the field of biomonitoring providing a device with acceptable stability, high sensitivity, good accuracy and precision.
KW - Electrochemical aptasensor (APT-MWCNT/GCE)
KW - Hydroxylated PCB (OH-PCBs)
KW - Multi-walled carbon nanotubes (MWCNTs)
KW - Serum analysis
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U2 - 10.1016/j.bios.2013.10.018
DO - 10.1016/j.bios.2013.10.018
M3 - Article
C2 - 24252763
AN - SCOPUS:84887590440
SN - 0956-5663
VL - 54
SP - 78
EP - 84
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -