Carbon nanotubes based electrochemical aptasensing platform for the detection of hydroxylated polychlorinated biphenyl in human blood serum

Sanaz Pilehvar, Jahangir Ahmad Rather, Freddy Dardenne, Johan Robbens, Ronny Blust, Karolien De Wael*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةArticleمراجعة النظراء

53 اقتباسات (Scopus)

ملخص

A novel strategy to sense target molecules in human blood serum is achieved by immobilizing aptamers (APTs) on multi-walled carbon nanotubes (MWCNT) modified electrodes. In this work, the aminated aptamer selected for hydroxylated polychlorinated biphenyl (OH-PCB) was covalently immobilized on the surface of the MWCNT-COOH modified glassy carbon electrode through amide linkage. The aptamers function as recognition probes for OH-PCB by the binding induced folding of the aptamer. The developed aptasensing device was characterized by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The aptasensor displayed excellent performance for OH-PCB detection with a linear range from 0.16 to 7.5μM. The sensitivity of the developed aptasensing platform is improved (1×10-8M) compared to the published report (1×10-6M) for the determination of OH-PCB (Turner et al., 2007). The better performance of the sensor is due to the unique platform, i.e. the presence of APTs onto electrodes and the combination with nanomaterials. The aptamer density on the electrode surface was estimated by chronocoulometry and was found to be 1.4×1013moleculescm-2. The validity of the method and applicability of the aptasensor was successfully evaluated by the detection of OH-PCB in a blood serum sample. The described approach for aptasensing opens up new perspectives in the field of biomonitoring providing a device with acceptable stability, high sensitivity, good accuracy and precision.

اللغة الأصليةEnglish
الصفحات (من إلى)78-84
عدد الصفحات7
دوريةBiosensors and Bioelectronics
مستوى الصوت54
المعرِّفات الرقمية للأشياء
حالة النشرPublished - أبريل 15 2014
منشور خارجيًانعم

ASJC Scopus subject areas

  • ???subjectarea.asjc.1300.1305???
  • ???subjectarea.asjc.1300.1304???
  • ???subjectarea.asjc.2200.2204???
  • ???subjectarea.asjc.1600.1603???

بصمة

أدرس بدقة موضوعات البحث “Carbon nanotubes based electrochemical aptasensing platform for the detection of hydroxylated polychlorinated biphenyl in human blood serum'. فهما يشكلان معًا بصمة فريدة.

قم بذكر هذا