Bioactive Constituents of Pulicaria jaubertii: A promising antihypertensive activity

Objective: Phytochemical investigation and evaluation of antihypertensive activity of the alcoholic extract of Pulicaria jaubertii aerial parts. Material and Methods: The chloroform and ethyl acetate fractions were chromatographed on silica gel and sephadex LH 20 to afford six compounds. Their structures were elucidated on the bases of extensive NMR (1H, 13C, DEPT, COSY, HSQC, HMBC, NOESY) and MS analysis. The antihypertensive activity of the alcoholic extract was evaluated against L-NAME-induced hypertensive rats. Results: One new monoterpene glucoside; (2R, 4S)-p-menth-1-ene-2-O-b-D-glucopyranoside [1] along with five known compounds; thymoquinol 2-O-b-D-glucopyranoside (zataroside-B) [2], quercetin 7, β-di-Omethylether (rhamnazin) [3], quercetin 7-O-methylether (rhamnetin) [4], quercetin β-O-methylether (isorhamnetin) [5] and stigmasterol 3-Ob-D-glucopyranoside [6] were isolated. The alcoholic extract significantly reduced the elevated SBP and partially restored the diminished nitric oxide metabolites. Conclusion: Compound [1] is a new and compounds [2-6] are reported for first time from Pulicaria jaubertii. The possible antihypertensive activity of the alcoholic extract may be due to its flavonoids and phenolic contents.