Application of a novel open-source program for measuring the effects of toxicants on the swimming behavior of large groups of unmarked fish

Michael J. Barry*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

24 اقتباسات (Scopus)

ملخص

The aim of this study was to demonstrate a novel method for measuring the effects of toxicants on the behavior of groups of unmarked fish. Ctrax is an open source Python-based machine vision program that was originally designed to track fruit flies (Drosophila). The effects of the fish anaesthetic quinaldine sulfate on the behavior of groups of 10 unmarked Arabian killifish (Aphanias dispar) in a small arena were measured. Ctrax was able to identify and track the X-Y coordinates of the individual fish over 1. min recording periods. The data was analyzed using specifically written Matlab (The Mathworks™) toolboxes and the effects of quinaldine sulfate on absolute swimming velocity, forward swimming velocity, rate of change in orientation and distance to wall were calculated. Additionally the effects of quinaldine sulfate on path tortuosity were also measured. Ctrax has significant benefits over other available technologies for tracking the 2-dimensional coordinates of fish. In particular the software is open source and thus freely available, it is accurate, requires only simple easily available equipment and able to track potentially large groups of fish (possibly >50) while maintaining their individual identities.

اللغة الأصليةEnglish
الصفحات (من إلى)938-944
عدد الصفحات7
دوريةChemosphere
مستوى الصوت86
رقم الإصدار9
المعرِّفات الرقمية للأشياء
حالة النشرPublished - مارس 2012

ASJC Scopus subject areas

  • ???subjectarea.asjc.2300.2305???
  • ???subjectarea.asjc.2300.2304???
  • ???subjectarea.asjc.1600???
  • ???subjectarea.asjc.2300.2310???
  • ???subjectarea.asjc.2300.2307???

بصمة

أدرس بدقة موضوعات البحث “Application of a novel open-source program for measuring the effects of toxicants on the swimming behavior of large groups of unmarked fish'. فهما يشكلان معًا بصمة فريدة.

قم بذكر هذا