TY - JOUR
T1 - Antioxidant and Antitumor Properties of Wild Blueberry (Sideroxylon mascatense)
T2 - Effects of Drying Methods
AU - Al Hasani, Shaima
AU - Al-Attabi, Zahir
AU - Waly, Mostafa
AU - Rahman, Mohammad
AU - Tamimi, Yahya
N1 - Publisher Copyright:
© 2021 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Background: Sideroxylon mascatense is a wild blueberry plant that traditionally known as 'boot' in Oman. Studies on health beneficial effects of this fruit are very limited. Aims and Objectives: This study aimed to evaluate the antioxidant and antitumor properties of Sideroxylon mascatense under different drying methods. Materials and Methods: Freeze (-20°C) and air drying (60 and 90°C) methods were used. The antioxidant potential properties were evaluated using DPPH and ABTS assay. Moreover, the antitumor activities of blueberry extracts were determined using Alamar blue assay. Results: The results of this study revealed that a blueberry extract at 20 mg powder/mL provided the maximum amount of vitamin C, reaching a value of 19.45 mg/100 g dry-basis in the flesh dried at 60°C and 14.24 mg/100 g dry-basis in seed dried at 90°C. The freeze-dried blueberry exhibited the highest percentage of free radical scavenging activity (DPPH) from both fruit flesh (50.8%) and seeds (39.4%) at a concentration of 20 mg/mL indicating a higher antioxidant activity. There was no significant difference in the ability of blueberry extracts to scavenge radical cations (ABTS+) at various concentrations of extracts. However, there was a 40% reduction of ABTS+radical formation in blueberry flesh extracts obtained from freeze-dried berry at a concentration of 20 mg/mL. On the other hand, blueberry seeds showed variation in the scavenging activity of the ABTS+radical of 38% after different processing methods. Breast MCF7 and ovarian Ov2008 tumor cell lines were the most sensitive to the dried blueberry. The 50% growth inhibition (IC50) of the freeze-dried blueberry extract at both cell lines was 64 and 69 μg/mL, respectively. Conclusion: Our findings indicated a potent antioxidant activity of Sideroxylon mascatense, but further research is required to isolate the active compounds in the fruit and test their effects in an experimental animal model.
AB - Background: Sideroxylon mascatense is a wild blueberry plant that traditionally known as 'boot' in Oman. Studies on health beneficial effects of this fruit are very limited. Aims and Objectives: This study aimed to evaluate the antioxidant and antitumor properties of Sideroxylon mascatense under different drying methods. Materials and Methods: Freeze (-20°C) and air drying (60 and 90°C) methods were used. The antioxidant potential properties were evaluated using DPPH and ABTS assay. Moreover, the antitumor activities of blueberry extracts were determined using Alamar blue assay. Results: The results of this study revealed that a blueberry extract at 20 mg powder/mL provided the maximum amount of vitamin C, reaching a value of 19.45 mg/100 g dry-basis in the flesh dried at 60°C and 14.24 mg/100 g dry-basis in seed dried at 90°C. The freeze-dried blueberry exhibited the highest percentage of free radical scavenging activity (DPPH) from both fruit flesh (50.8%) and seeds (39.4%) at a concentration of 20 mg/mL indicating a higher antioxidant activity. There was no significant difference in the ability of blueberry extracts to scavenge radical cations (ABTS+) at various concentrations of extracts. However, there was a 40% reduction of ABTS+radical formation in blueberry flesh extracts obtained from freeze-dried berry at a concentration of 20 mg/mL. On the other hand, blueberry seeds showed variation in the scavenging activity of the ABTS+radical of 38% after different processing methods. Breast MCF7 and ovarian Ov2008 tumor cell lines were the most sensitive to the dried blueberry. The 50% growth inhibition (IC50) of the freeze-dried blueberry extract at both cell lines was 64 and 69 μg/mL, respectively. Conclusion: Our findings indicated a potent antioxidant activity of Sideroxylon mascatense, but further research is required to isolate the active compounds in the fruit and test their effects in an experimental animal model.
KW - Antioxidants
KW - cancer
KW - drying methods
KW - sideroxylon mascatense
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U2 - 10.4103/ijnpnd.ijnpnd-76-20
DO - 10.4103/ijnpnd.ijnpnd-76-20
M3 - Article
AN - SCOPUS:85101390850
SN - 2231-0738
VL - 11
SP - 71
EP - 79
JO - International Journal of Nutrition, Pharmacology, Neurological Diseases
JF - International Journal of Nutrition, Pharmacology, Neurological Diseases
IS - 1
ER -