TY - JOUR
T1 - Anti-bacterial activity of cow urine extract of neem against Xanthomonas Okyzae-the causative agent of bacterial leaf blight disease of paddy
AU - Shanthi, Subbarayan
AU - Sivakumar, Nallusamy
AU - Rajapandian, K.
AU - Panneerselvam, Annamalai
AU - Radha, Natarajan
N1 - Publisher Copyright:
© Global Science Publications.
PY - 2015
Y1 - 2015
N2 - Bactericidal effects of different extracts of cow urine and neem (Azadirachta indica A. Juss) was studied against Xanthomonas oryzae pv oryzae, the causative agent of bacterial leaf blight disease of paddy. Fresh, incubated, condensed and chloroform extracts of cow urine were tested against X. oryzae pv oryzae. Similarly aqueous, hexane, chloroform, ethyl acetate, methanol, alcohol and cow urine extracts of A. indica were tested for their anüxanthomonas activity. Cow urine extract of A. indica was more effective than the other extracts. Maximum antibacterial activity of cow urine extract of A. indica against X. oryzae pv oryzae was observed at 1600 μg concentration. Phytochemical analysis of incubated cow urine extract of A. indica showed the presence of flavonoids, quinines, alkaloids, coumarin, tannin, saponin and phenol. The GC-MS and FT-ER studies revealed the presence of oxiranes, dibutyl thalate, phenol, benzene dicarboxylic acid which could be the possible essential antimicrobial principles against X. oryzae.
AB - Bactericidal effects of different extracts of cow urine and neem (Azadirachta indica A. Juss) was studied against Xanthomonas oryzae pv oryzae, the causative agent of bacterial leaf blight disease of paddy. Fresh, incubated, condensed and chloroform extracts of cow urine were tested against X. oryzae pv oryzae. Similarly aqueous, hexane, chloroform, ethyl acetate, methanol, alcohol and cow urine extracts of A. indica were tested for their anüxanthomonas activity. Cow urine extract of A. indica was more effective than the other extracts. Maximum antibacterial activity of cow urine extract of A. indica against X. oryzae pv oryzae was observed at 1600 μg concentration. Phytochemical analysis of incubated cow urine extract of A. indica showed the presence of flavonoids, quinines, alkaloids, coumarin, tannin, saponin and phenol. The GC-MS and FT-ER studies revealed the presence of oxiranes, dibutyl thalate, phenol, benzene dicarboxylic acid which could be the possible essential antimicrobial principles against X. oryzae.
KW - Azadirachta indica
KW - Bacterial leaf blight disease
KW - Cow urine extract
KW - Xanthomonas oryzae pv oryzae
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M3 - Article
AN - SCOPUS:84930318892
SN - 0972-3005
VL - 17
SP - 167
EP - 175
JO - Asian Journal of Microbiology, Biotechnology and Environmental Sciences
JF - Asian Journal of Microbiology, Biotechnology and Environmental Sciences
IS - 1
ER -