TY - JOUR
T1 - Analysis of phenols in water by high-performance liquid chromatography using coumarin-6-sulfonyl chloride as a fluorogenic precolumn label
AU - Suliman, Fakhr Eldin O.
AU - Al-Kindi, Suad S.
AU - Al-Kindy, Salma M.Z.
AU - Al-Lawati, Haider A.J.
PY - 2006/1/6
Y1 - 2006/1/6
N2 - A simple, sensitive and rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method is proposed for the analysis of some environmentally important phenols in water. The use of coumarin-6-sulphonyl chloride (C6SCl) as a fluorescence-labeling reagent has been investigated. The compound reacts with phenols within 20 min under mild conditions (ambient temperature, pH 9.0) to give sulphonates that can be separated by RP-HPLC employing fluorescence detection at λex = 360 and λem = 460 nm. The optimum conditions for fluorescence, derivatization and chromatographic separation have been established and detection limits in the range 0.1-0.9 μg l-1 were obtained for the studied compounds. The calibration curves were linear for the range 6-200 μg l-1 for phenol, 3-200 μg l-1 for 2-chlorophenol, 4-chlorophenol and 2,3,5-trichlorophenol and for the range of 3-100 μg l -1 for 2,3-dichlorophenol and 3,5-dichlorophenol. The practical applicability of the method to environmental samples was demonstrated by analyzing drinking and industrial water samples spiked with the phenolic compounds.
AB - A simple, sensitive and rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method is proposed for the analysis of some environmentally important phenols in water. The use of coumarin-6-sulphonyl chloride (C6SCl) as a fluorescence-labeling reagent has been investigated. The compound reacts with phenols within 20 min under mild conditions (ambient temperature, pH 9.0) to give sulphonates that can be separated by RP-HPLC employing fluorescence detection at λex = 360 and λem = 460 nm. The optimum conditions for fluorescence, derivatization and chromatographic separation have been established and detection limits in the range 0.1-0.9 μg l-1 were obtained for the studied compounds. The calibration curves were linear for the range 6-200 μg l-1 for phenol, 3-200 μg l-1 for 2-chlorophenol, 4-chlorophenol and 2,3,5-trichlorophenol and for the range of 3-100 μg l -1 for 2,3-dichlorophenol and 3,5-dichlorophenol. The practical applicability of the method to environmental samples was demonstrated by analyzing drinking and industrial water samples spiked with the phenolic compounds.
KW - Cuomarin-6-sulfonyl chloride
KW - Fluorescence
KW - HPLC
KW - Phenols
UR - http://www.scopus.com/inward/record.url?scp=28944442326&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=28944442326&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2005.09.094
DO - 10.1016/j.chroma.2005.09.094
M3 - Article
C2 - 16266712
AN - SCOPUS:28944442326
SN - 0021-9673
VL - 1101
SP - 179
EP - 184
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -