TY - JOUR
T1 - Amplification and sequencing of Brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in Austrian pigs shows frequent solitary presence of Brachyspira murdochii
AU - Weissenböck, Herbert
AU - Maderner, Anton
AU - Herzog, Anna Maria
AU - Lussy, Helga
AU - Nowotny, Norbert
N1 - Funding Information:
We thank Gudrun Kurz for excellent technical assistance and Klaus Bittermann for professional digital artwork. This study was partially funded by the Hochschuljubiläumsstiftung of the City of Vienna. We are grateful to Tim K. Jensen and Ronny Lindberg, who provided paraffin-embedded tissue samples of Br. hyodysenteriae infected pigs as positive controls.
PY - 2005/11/30
Y1 - 2005/11/30
N2 - Brachyspira infections are significant causes of enterocolitis in pigs. In order to differentiate pathogenic species (Brachyspira (Br.) hyodysenteriae, Brachyspira pilosicoli) from less pathogenic or non-pathogenic species (Brachyspira intermedia, Brachyspira innocens, Brachyspira murdochii) in paraffin-embedded tissue samples a polymerase chain reaction (PCR) protocol allowing identification of Brachyspira at species level in archival material was developed. This approach was complemented by sequencing of the PCR amplification products. All seven cases presented with clinical and morphological Brachyspira-associated enterocolitis. Br. hyodysenteriae was not identified in any of the cases, while Br. pilosicoli was identified in a single case in conjunction with Br. murdochii. One case each was found positive for Br. innocens and Br. intermedia. Interestingly, the majority of cases presented as single or double infections with Br. murdochii. In some of the pigs other pathogens, like porcine circovirus-2 or Lawsonia intracellularis were present. These observations point at the possibility that under certain conditions even Brachyspira species of low pathogenicity can multiplicate extensively and lead to Brachyspira-associated enterocolitis.
AB - Brachyspira infections are significant causes of enterocolitis in pigs. In order to differentiate pathogenic species (Brachyspira (Br.) hyodysenteriae, Brachyspira pilosicoli) from less pathogenic or non-pathogenic species (Brachyspira intermedia, Brachyspira innocens, Brachyspira murdochii) in paraffin-embedded tissue samples a polymerase chain reaction (PCR) protocol allowing identification of Brachyspira at species level in archival material was developed. This approach was complemented by sequencing of the PCR amplification products. All seven cases presented with clinical and morphological Brachyspira-associated enterocolitis. Br. hyodysenteriae was not identified in any of the cases, while Br. pilosicoli was identified in a single case in conjunction with Br. murdochii. One case each was found positive for Br. innocens and Br. intermedia. Interestingly, the majority of cases presented as single or double infections with Br. murdochii. In some of the pigs other pathogens, like porcine circovirus-2 or Lawsonia intracellularis were present. These observations point at the possibility that under certain conditions even Brachyspira species of low pathogenicity can multiplicate extensively and lead to Brachyspira-associated enterocolitis.
KW - Austria
KW - Brachyspira
KW - Colitis
KW - Genotyping
KW - PCR
KW - Paraffin-embedded tissue
UR - http://www.scopus.com/inward/record.url?scp=27644542649&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27644542649&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2005.09.002
DO - 10.1016/j.vetmic.2005.09.002
M3 - Article
C2 - 16213113
AN - SCOPUS:27644542649
SN - 0378-1135
VL - 111
SP - 67
EP - 75
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 1-2
ER -