TY - JOUR
T1 - Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells
AU - Venkatesh Gobi, Veerappan
AU - Rajasankar, Srinivasagam
AU - Ramkumar, Muthu
AU - Dhanalakshmi, Chinnasamy
AU - Manivasagam, Thamilarasan
AU - Justin Thenmozhi, Arokiasamy
AU - Essa, Musthafa Mohamed
AU - Chidambaram, Ranganathan
N1 - Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2018/2/7
Y1 - 2018/2/7
N2 - The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson’s disease (PD).
AB - The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson’s disease (PD).
KW - A. blazei
KW - Apoptosis
KW - Mitochondria membrane potential
KW - Oxidative stress
KW - Rotenone
UR - http://www.scopus.com/inward/record.url?scp=84988362885&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84988362885&partnerID=8YFLogxK
U2 - 10.1080/1028415X.2016.1222332
DO - 10.1080/1028415X.2016.1222332
M3 - Article
C2 - 27646574
AN - SCOPUS:84988362885
SN - 1028-415X
VL - 21
SP - 97
EP - 107
JO - Nutritional Neuroscience
JF - Nutritional Neuroscience
IS - 2
ER -