Effect of Dipeptidyl peptidase inhibtot (Sitagliptin) and sodium glucose co transporter (SGLT2) inhibitor (canaglifozin) on experimental animal models of acute renal injury and chronic kidney disease

  • Ali, Badreldin (PI)

المشروع: Other project

تفاصيل المشروع

Description

This work aims at investigating the possible ameliorative effects of two Dipeptidyl peptidase ? 4 (DPP4) inhibitors (sitagliptin and angagliptin), and two sodium glucose co-transporter (SGLT2) inhibitors (empagliflozin and dapagliflozin) on gentamicin ? induced acute renal injury (ARI) and adenine ? induced chronic kidney disease (CKD) in rats. In the studies on ARI, the test drugs will be given orally for 15 consecutive days, and on the 10th day, some of the treated rats were also injected intraperitoneally with either saline or gentamicin (80 mg/kg, day for 5 days). CKD will be induced by giving the rats adenine (0.25% in the feed, 5 weeks). Different doses of the test drugs will be given by oral gavage concomitantly with adenine. In all experiments, 24-h before sacrifice, urine will be collected from all rats, which will then be anaesthetized for blood pressure and renal blood flow monitoring. This will be followed by intravenous injection of norepinephrine for the assessment of renal vasoconstrictor responses. Thereafter, blood and kidneys will be collected for measuring several physiological, biochemical, molecular, histopathological and histochemical parameters. In separate groups, we will also carry out myograph studies, to assess the vascular reactivity of isolated vessels by comparing concentration-response curves of carbachol and phenylephrine among all the treated groups in the two sets of experiments. The concentration of gentamicin in the cortical tissues will also be measured in the ARI experiments, as well as fasting blood glucose concentrations at frequent intervals in all experiments.

Layman's description

This work aims at investigating the possible ameliorative effects of two Dipeptidyl peptidase ? 4 (DPP4) inhibitors (sitagliptin and angagliptin), and two sodium glucose co-transporter (SGLT2) inhibitors (empagliflozin and dapagliflozin) on gentamicin ? induced acute renal injury (ARI) and adenine ? induced chronic kidney disease (CKD) in rats. In the studies on ARI, the test drugs will be given orally for 15 consecutive days, and on the 10th day, some of the treated rats were also injected intraperitoneally with either saline or gentamicin (80 mg/kg, day for 5 days). CKD will be induced by giving the rats adenine (0.25% in the feed, 5 weeks). Different doses of the test drugs will be given by oral gavage concomitantly with adenine. In all experiments, 24-h before sacrifice, urine will be collected from all rats, which will then be anaesthetized for blood pressure and renal blood flow monitoring. This will be followed by intravenous injection of norepinephrine for the assessment of renal vasoconstrictor responses. Thereafter, blood and kidneys will be collected for measuring several physiological, biochemical, molecular, histopathological and histochemical parameters. In separate groups, we will also carry out myograph studies, to assess the vascular reactivity of isolated vessels by comparing concentration-response curves of carbachol and phenylephrine among all the treated groups in the two sets of experiments. The concentration of gentamicin in the cortical tissues will also be measured in the ARI experiments, as well as fasting blood glucose concentrations at frequent intervals in all experiments.
اختصارTTotP
الحالةلم يبدأ

بصمة

استكشف موضوعات البحث التي تناولها هذا المشروع. يتم إنشاء هذه الملصقات بناءً على الجوائز/المنح الأساسية. فهما يشكلان معًا بصمة فريدة.